SCMV Related Genes in the
Cultured Stealth-Adapted Virus
CMV-Related Matching
of Stealth Virus Sequences to
Contiguous SCMV
BaCMV RhCMV HCMV
Sequences nucleotide
matching (Expect value)
Genes
Length
UL
14 1,458
UL
28-48 28,199
UL
48-54 8,407 UL50
571/598 422/465 384/453 159/185
UL
56 767
(0.0) (e-180) (e-95)
(e-38)
UL 57 +
ori 2,748 UL57 1226/1246
499/577 Not avail. 370/492
UL
61-69 4,459
(0.0)
(0.0) (e-62)
UL
70 1,729
UL
71-76 6,328
UL
77-78 1,884
UL
84-104 25,023 UL93 630/656
575/647 368/406 266/317
UL
104-105 1,464
(0.0) (0.0)
(e-137) (e-57)
UL
111-112 1,955 UL111 760/807
709/901 74/89 75/89
UL
115-132 8,628
(0.0) (e-171)
(e-7) (e-15)
UL
141-144 5,820
US
20-29 16,011 *5 copies of US28 related
gene: a chemokine (and also
HIV) receptor.
US
30-32 3,978
Analysis
based on >300 clones of DNA of stealth virus
culture. There is good evidence that the virus has a
fragmented, unstable genome. Note apparent absence
of UL 83 and UL 55. The UL 123 showed significant
mutations. The products of these three genes would
ordinarily provide the major antigens recognized by
anti-CMV cytotoxic T cells. The nucleotide homology
data establish that this particular stealth-adapted
virus was derived from SCMV. It most likely arose
from an SCMV-contaminated batch of live polio virus
vaccine.
Analysis
of the Bacteria Derived Genes
Clones
obtained Bacterium
showing Nucleotide Bacterial
proteins/genes
identified
by sequence
from
stealth virus the highest level of (amino
acid) homology to the cloned
DNA
obtained from the
SCMV
derived stealth
virus culture
culture
that match sequence homology identity
to bacterial
genes
Clone Size Expect value
3B43 3,620 Brucella melitensis
0.0 ribosomal RNA operon C
3B23
8,916
" 0.0
sorbose dehydrogenase,
hippurate
hydrolase
3B313
7,985
" 0.0
ABC transporter, metal
chelation,
secreted protein
3B614
5,062
" 0.0
acetyl-CoA carboxylase,
dehydroquinate
shikimic acid
kinase, recombinase
3B534 612
3e-101 enolase
3B315 4,495
" (4e-97)
glutathione S-transferase,
transcriptional
regulator
C16134
4,142 "
0.0 dihydrodipicolinate
synthase,
guanosine
pyrophosphohydrolase, DNA
to RNA polymerase
C1616 4,626
" e-120
UDP-epimerase, 3-
demethylubiquinone-9-3
methyltransferase,
sodium/bile cotransporter,
transposon from plasmid of Agrobacterium
(0.0)
3B41 2,869 Agrobacterium tumefaciens (2e-155)
sensory transduction histidine kinase
3B47
2,024 Sinorhizobium meliloti (8e-139)
ABC transporter,
C16122
4,915 Zymomonas mobilis (5e-63)
dihydrodipicolinate synthase,
cystathionine
gamma-synthase
3B513 8,106 Alpha-proteobacteria sp.
(e-30) Fe binding, nitrogen fixating, invertase,
others
3B512
2,345 Mycoplasma (5e-98)
ATP-transporter p115-like
3B520 2,797
" (e-37)
ABC transporters p29 and p69
3B35
2,142
" (5e-81)
unknown function
3B632
1,396 Streptococcus
(8e-33) ribonuclease H
3B528
2,049
" (3e-33)
glucuronyl hydrolase, sugar transporter
Conclusion: Modified
bacteria-derived sequences present in stealth virus
culture. For many proteins there are structural
similarities with related proteins from other
bacterial species, leading to the probability of
antigenic cross-reactivity.
